dextran hydrodynamic radius

2012;3(1):208. Kasaai, M. R.; Arul, J.; Charlet, G. (2000). TEWL measurements were performed before and 30min after microporation. Determination of branching in biopolymers by size exclusion chromatography with light scattering (Malls), viscosity, and refractive index detection. The values of Mark-Houwink parameters universalized for the range of working temperatures are the following: 0.4267 for a*, and 0.1455 cm3/g for k*M-H, with 2 of 0.9399. Keywords: FITC dextran; intestinal permeability; transit time. In addition, the permeation rate was higher in pig skin than in human skin. These studies were carried out at the Hisko (Gunpo, Korea). The relation between Mw and the intrinsic viscosity is given by Mark-Houwink equation. Microporation applications for enhancing drug delivery. Eremeeva, T. E.; Bykova, T. O. Drug deliv. Figure1 shows a schematic illustration of transdermal delivery after the application of the microporation device. ; Tsuchiya, H.M.; Rist, C.E. Masuelli, Martin Alberto (2013). Rheology of dextran solutions. The hydrodynamic radius R h, defined as the radius of a hard sphere that diffuses at the same rate as the solute, takes these effects into account. 20K groups (n=3, *: p<0.05, **: p<0.01). dextran hydrodynamic radius. Int J Food Sci Nutr. (1987). Saudi Pharm J. The genus Weissella: taxonomy, ecology and biotechnological potential. The micropore formed at the SC through to the epidermis, enabling drug delivery. Webdextran hydrodynamic radius. Mitragotri S. Mechanical disruption of skin barrier for vaccine delivery. Dynamics of a Polymer Solution in a Rigid Matriz. Huggins, Maurice L. (1942). 2013 May;64(3):346-54. doi: 10.3109/09637486.2012.734289. Conducting a comparative analysis of the data shown in Table 1 can observer that parameter data Mark-Houwink a, obtained by these authors are very similar to that obtained in this work, which affirms its validity. The human and pig skin samples were placed in the receiver chamber of the Transdermal diffusion cell system with the SC facing up and the donor chamber was fixed in place. Table 2 compares the properties of nanoparticles coated with one or other polymer. Hydrodynamic cavitation (HC), a common phenomenon that can produce local excessive temperature, high pressure and large amount of energy. To overcome these limitations, microporation devices that act as therapeutic vehicles for delivering drug molecules across the skin barriers have been developed. 4h after microporation, the sizes of the micropore decreased slightly. Experimental Review on Intrinsic Viscosity Measurements and Temperature Effect.". Nat Biotechnol. Clipboard, Search History, and several other advanced features are temporarily unavailable. This study was supported by the LG Electronics. Just in case anyone wants to use a different molecular weight to estimate the hydrodynamic size: the calculator is integrated into the classic Zeta (2008). ; Dutt, N.V.K. Although, in literature there is much information on hydrodynamic measurements from determinations viscosity; very few of them evaluate the situation at different temperatures. (2013). Progress in Polymer Science, 30, 38-70. 2008;26(11):12618. Table 2 compares the properties of nanoparticles coated with one or other polymer. The term f/f0 is sometimes denoted as P, Perrin constant. WebDextran produced from Weissella cibaria JAG8 was purified and characterized. 56 The linkage that the enzyme dextranase breaks down is indicated by the black arrow. For a given polymer-solvent pair, the theta condition is satisfied at a certain temperature, called the theta () temperature or theta point. The value of P increases from 1.0272 to 1.2694 (spherical shape with tendency to spheroid for high Mw). The explanation for the anomalous values of a and kM-H is because the mixture solute-solvent is highly compatible due a great compaction of biopolymer, from undisturbed state in the absence of interactions, where free energy of mixing less than zero, and emphasize the nature non-linear or hyperbranched of dextran for Mw high to 200kDa. Molecular weight, particle size and diffusion coefficient can be determined in many studies by Dynamic Light Scattering [20, 21, 22], Gel Permeation Chromatography [23], Analytical Ultracentrifuge [24, intrinsic viscosity [1, 7, 14], size exclusion chromatography / multiangle laser light scattering / differential viscometry, Flow Field-Flow Fractionation [25], and so on. With FITC-dextran 20K, cumulative permeation in the human skin was 2.530.48M, and 3.340.19M in the pig skin, which significantly lower than FITC-dextran 4K. Cumulative amount of FITC-dextran permeated after microporation on the human and pig skin for FITC-dextran M.W.. a M.W. In vitro studies with human cadaver skin is a viable, ethical, and economical alternative for in vivo animal studies. Advances in chemistry series Washington, DC: American Chemical Society., pp. a Immediately after microporation, b 4h, c 8h, and d 24h (n=5, *: p<0.05). Teixeira CG, Fusieger A, Milio GL, Martins E, Drider D, Nero LA, de Carvalho AF. All possible degrees of curling can be displayed by any molecule, but there will be an average configuration which will depend on the solvent. The particular dextrans which were used initially for conversion into synthetic blood-volume expanders [6, 7], human red blood cells aggregation for increasing the degree of polymerization and hence the molecular weight [8, 9, 10], hydrogels and microspheres [11], in drug transport system and its modifications as nanoparticles [12], in the removal through absorption of contaminant effluents such as heavy metals, organic molecules and inks [13], in the formation of biodegradable films [14, 15]. Transdermal delivery is of great importance for the effective delivery of bioactive or therapeutic agents into a body. A few percent of R (13) glucosidic linkages provides side chains which appear to be short. Table 5 shows the data of Mark-Houwink parameters determined in figure 4 from linear regressions with 2 values higher than 0.9797. Progress in Biophysical Molecules Biological, 68, 207-262. Chem. For a sphere and a random coil 0 the values are 9.23x1023 mol-1 and 2.60x 1023mol-1, respectively. Hrabchak C, Flynn L, Woodhouse KA. Journal of Physical Chemistry. The Dextrans intrinsic viscosity is determined as a function of the molecular weight in water and NaOH 0.5 M see reference [1]. If there are particles in the sample, the incident laser light gets scattered in all directions. The reason for a nonlinear Mark-Houwink relationship is confirmed by the intrinsic viscosity which in contrast with scaling prediction shows no power law behavior. Lucifer yellow (LY) and FITC-labelled dextran stock solutions were prepared in Ringers-HEPES buffer (150 mM NaCl, 3.4 mM CaCl 2, 1.2 mM MgCl 2, 5.2 mM KCl, 0.5 mM NaHCO 3, 2.8 mM glucose, and 10 mM HEPES) and frozen at 20C. The solutions were prepared in bidestilated water at 1, 0.75, 0.5, 0.25 % wt. Treatment of acne scars by fractional bipolar radiofrequency energy. Home > 2022 > Junho > 22 > Uncategorized > dextran hydrodynamic radius. When applied to adherent cell cultures, the APGD-t is capable of inducing temporary cell permeabilization. Synthesis of water-soluble and water-insoluble amphiphilic derivatives of dextran in organic medium. Carbohydrate Polymers 72, 356-360. The skin was treated with the RF-based microporation device, inducing micropore ablation through alternating electrical current. For data requests, please contact the authors. Journal of Controlled Release, 119, 5-24. 2012 Sep 1;90(1):441-6. doi: 10.1016/j.carbpol.2012.05.063. Journal of Physical Chemistry, 58(11), 953-957. The most widely used method for the characterization of macromolecules is the capillary viscometry, as it is a simple and economic method. ; Hogan, M.P. The Mark-Houwink a constant is close to 0.5 in poor solvents. Viscosity, sedimentation, and light-scattering properties of fraction of an acidhydrolyzed dextran. 7 8 Synthesis of dextran by dextransucrase treated, sucrose-supplemented skimmed milk revealed the promising potential of dextran as a food additive. 1905/05) for their financial support. WebIn this work, hydrodynamic properties are investigated in different dextran aqueous solutions with different molecular weights (8.8-2000kDa), in a temperature range from 20C to 50C. Biodegradable dextran hydrogels crosslinked by stereocomplex formation for the controlled release of pharmaceutical proteins. The receptor phase was collected immediately (time 0) and 2, 4, 8, 12, and 24h. This receptor phase solution was transferred to a 96-well plate and the skin-permeated FITC-dextran was measured using a microplate reader (Molecular Devices, Co. Ltd., Sunnyvale, CA, USA) at 405nm. 2003;10:33847. Viscotek GmbH, Europe. 2013;43(4):287303. In general, Mv is not experimentally accessible, whereas other average molecular weights are accessible. Please enable it to take advantage of the complete set of features! Carbohydrate Polymers 88 373-381. A solvent at this temperature is called a theta solvent [53]. Lpez Martnez, M. C.; Daz Baos, F. G.; Ortega Retuerta, A.; Garca de , J. Sachdeva V, Banga AK. Banga AK. 2017 Dec 12;27(1):73-78. doi: 10.1007/s10068-017-0274-0. All branched polysaccharide types could be described approximately by the hyperbranching theory. 113, 81-86. Its Invariancy with Respect to the Cross-Sectional Dimensions of Polymer Chains. (2000). Accessibility Interaction Parameter (); Expansion Factor (); Steric Hindrance Factor (); and Shielding Function F(); for the System PEAOrganic Solvents by Intrinsic Viscosity Measurements. Dextran produced from Weissella cibaria JAG8 was purified and characterized. The column was eluted with bidestilled water a flow rate of 0.8 ml/min, injection 5L, back pressure 200psi. Pore closure examination was performed using an optical microscope (BX43, Olympus, Japan) immediately (time 0), 4, 8, and 24h following device application. Monolayers and Thin Films of Dextran Hydrophobically Modified. We will also conduct further studies on the regeneration of human cadaver skin and evaluate the possibilities of clinical trials. 2.It can be found from the figure that at the start-up stage of the shaft, the hydrodynamic effect (HE) is weak, so the HP is not enough to support the shaft, which The Mark-Houwink. Named after George Gabriel Stokes, it is closely related to solute mobility, factoring in not only size but also solvent effects.A smaller ion with stronger hydration, for example, may have a greater Stokes radius than a larger ion with ; Bos, G.W. Ind. Polyelectrolytes as secondary calibration standards for aqueous SEC. Advances in Cosmetic Surgery. These studies on Mark-Houwink parameters are usually carried out at a given temperature, obtaining a consistent result but in a very limited temperature range [68]. Many works are aimed at determining molecular weights [1, 7, 26], hydrodynamic radius, among other parameters for characterizing the physics and chemistry properties of dextran in solution. The calculation of Mark-Houwink parameters is carried out by the graphic representation of the following equation: where kMH (cm3/g) and a are Mark-Houwink constants, depending on the type of polymer, solvent, and temperature of intrinsic viscosity determinations [46]. sharing sensitive information, make sure youre on a federal The relative browning for dextran from W. cibaria JAG8 was similar to commercial prebiotic Nutraflora P-95 and 3-fold lower than Raftilose P-95. Multiple Linear Least-Squares Fits with a Common Intercept: Determination of the Intrinsic Viscosity of Macromolecules in Solution. Alternatively, the value of qM-H can be calculated using numerical methods and other-average molecular weights (Mn, Mw, Mz) according to where c and d are empirical polynomial functions of the exponent a. ; Patel, T.R. WebHydrodynamic radius = variable size of an analyte with specific MW based on dynamical properties. Kam Y, Sacks H, Kaplan KM, Stern M, Levin G. Radio frequency-microchannels for transdermal delivery: characterization of skin recovery and delivery window. The permeability of fluorescein isothiocyanate-dextrans (FITC-dextrans) with different molecular weights through the pig and human cadaver skins were measured using Franz diffusion cell. European Polymer Journal, 37, 619-622. 10% neutral buffered formalin was purchased from Hisko (Gunpo, Korea). Proc of SPIE. These results are in agreement with a previous study which suggested that the microporation using radiofrequency causes disruption of the SC, enabling the percutaneous permeation of the FITC-dextran [21]. Dextran molecular size and degree of branching as a function of sucrose concentration, pH, and temperature of reaction of. Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. The molecular weight and Simha number do not change in this temperature range (Mw from 8.8-200kDa), P slightly change, showing modifications in the hydrodynamic properties of the biopolymer in aqueous solution as [] and RH. Markiertes Dextran wird hufig per Mikroinjektion in die Zellen eingebracht. Ultimately they concluded that the temperature increment found to be an effective force in disturbing these interactions, mainly between polymer-polymer and polymer-solvent molecules for the used system. European Polymer Journal, 36, 2143-2146. where tr is the column retention time in minutes (2 of 0.9792). The basic setup of a DLS instrument is shown in Figure 1. Hydrodynamic radii ( RH) were determined by dynamic light scattering. 2016;9706:97061E6. The receptor chamber was filled with PBS. WebThe study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Various average molecular weights (Mv, Mn, Mw, and Mz) instead of Mv were substituted in M-H equation. Pavlov, G.M. To improve drug permeability, micropore ablation using RF is performed by passing an alternating electrical current through the skin at a frequency higher than 100kHz. The value of qM-H varies from one sample to another one, because it is a function of a and the average molecular weights (Mv, Mw). Dextran; Weissella cibaria; exopolysaccharide; prebiotic; skimmed milk. Amersham Biosciences, AA Edition, www.amershambiosciences.com. Journal of Polymer Science Part B: Polymer Physics, 38, 2591-2598. FITC-derivatives have been used for a variety of biological applications for more than 40 yeras. This conjecture found to be correct. Polysaccharide hydrogels for modified release formulations. ACS Nano. The polymer molecule assumes a tighter configuration, and the solution has a lower intrinsic viscosity. On the molecular weight distribution of dextran T-500. Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. The solution viscosity () is related to the solvent viscosity (o), resulting in the relative viscosity. The hydrodynamic 57 radius of the dextranase is 7 WebDextran 670 GPC Standard Normative Mw - 670000 Da GPC standards can be used for calibration and molecular weight determination. The study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. Molecular weight determination of polysaccharides. Results and Discussion The microscopic dynamics of proteins are probed in a light scattering experiment. Senti, F. R.; Hellman, N. N.; Ludwig, G.; Babcock, E.; Tobin, R.; Class, C. A., (1955). Mark-Houwink-Kuhn-Sakurada Exponent at the Condition. ; Ortega, A.; Garcia de la Torre, J.; Harding, S.E. 2018 Jan;184(1):1-11. doi: 10.1007/s12010-017-2522-4. Google Scholar. After the pore closure examination, each sample was stored in a 10% neutral buffered formalin awaiting haematoxylin/eosin (H&E) staining. Human cadaver skin has been confirmed as alive skin tissue through analysis of its morphology and enzymes, and its usefulness as an alternative skin membrane in drug permeation experiments has been verified [20]. Therefore, it is suitable as model molecules with different molecular weights for transdermal drug delivery [29]. (1937). Where f0 is the frictional coefficient of a sphere having the same hydrodynamic (hydrated or solvated) volume V as the particle. Webprotein hydrodynamic radius was observed with increasing protein concentration (in the range 0.25-2 mM) in both the absence and presence of 8 M urea. Drug Deliv. These studies were again focused on investigating the hydrodynamic properties of the polymer molecules, their hydrodynamic radius in relation to their radius of gyration and the coherence of the simulation results with polymer theory. @ Nilimesh See attached. Transdermal drug delivery: overcoming the skins barrier function. A sugar cane native dextran as an innovative functional excipient for the development of pharmaceutical tablets. 4K, 10K, and 20K) permeated through the human, and pig skin after microporation. Measurements were made with a Ubbelohde C type suspended level viscometer [57] (IVA, Argentina); with a diameter capillary of 0.81mm and water draining time of 35.89s at 20C, with viscometer constant of 0.027883 mm2/s [58]. Chem. We offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges. Typical examples are the classical size-independent combinations like the Flory parameter, which that combine the intrinsic viscosity, [], and the gyration radius, Rg: These quantities have been proposed along the years, by different eminent scientists, after whom they are named.

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